Journal: Applied Microbiology and Biotechnology

Article Title: Recent advances in the potential applications of luminescence-based, SPR-based, and carbon-based biosensors

doi: 10.1007/s00253-022-11901-6

Figure Lengend Snippet: Advances and potential application of biosensors in pharmaceutical sciences

Article Snippet: Electrochemical immunosensor , Zika virus (envelope protein) , 10 pM , Zika virus (strain Zika SPH2015) envelope protein (ZIKV-E) ELISA Pair Set; Sino Biological Inc. (LOD = 125 pg/mL) , Promising clinical application for early-stage diagnostics of the virus, operation time around 40 min , Kaushik et al. ( ) .

Techniques: Förster Resonance Energy Transfer, Imaging, SPR Assay, Electrochemiluminescence, MicroChIP Assay, In Vitro, Infection, Amplification, Isolation, Biomarker Assay, Sequencing

Journal: Applied Microbiology and Biotechnology

Article Title: Recent advances in the potential applications of luminescence-based, SPR-based, and carbon-based biosensors

doi: 10.1007/s00253-022-11901-6

Figure Lengend Snippet: Summarization of LOD, advances of the abovementioned biosensors, and their comparison with common methods of detection

Article Snippet: Electrochemical immunosensor , Zika virus (envelope protein) , 10 pM , Zika virus (strain Zika SPH2015) envelope protein (ZIKV-E) ELISA Pair Set; Sino Biological Inc. (LOD = 125 pg/mL) , Promising clinical application for early-stage diagnostics of the virus, operation time around 40 min , Kaushik et al. ( ) .

Techniques: Glutamate Assay, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Sequencing, Simultaneous Assay, Biomarker Assay, Fluorescence, In Situ Hybridization

Journal: NPJ Vaccines

Article Title: Immunization-induced antigen archiving enhances local memory CD8+ T cell responses following an unrelated viral infection

doi: 10.1038/s41541-024-00856-6

Figure Lengend Snippet: a Experimental schematic for b – e . C57/BL6 mice were immunized subcutaneously in the footpad and/or flank with the indicated antigens and adjuvants. b Cells were stained with CD45, PDPN, CD31 and PD-L1. Cells were gated on CD45-PDPN + CD31- for FRC and CD45-PDPN + CD31+ for LECs. Shown are examples of LEC and FRC antigen-positive cells based on PD-L1 expression (floor, MARCO LEC) and ova-AF488+ from mice 2–3 weeks after immunization with ova conjugated to Alexa-Fluor 488 (AF488) and polyI:C and αCD40. c Quantification of the frequency of LEC, BEC, and FRC in the popliteal lymph node (pLN) that are positive for the indicated antigens administered with polyI:C and αCD40 at indicated time. d Same as ( b ) except for mice were immunized with SARS-CoV-2-RBD-AF488, polyI:C, and αCD40. e Same as in ( c ) except for SARS-CoV-2-RBD and CHIKV-E2 with polyI:C and αCD40. CHIKV-E2 was repeated for 9–14 days post-vaccine (~2 weeks). Statistical analysis was done using an unpaired t -test where the p -value between naïve and indicated antigen is <0.0001. In each experiment, at least n = 2–3 mice per group were evaluated and the experiment was repeated n = 2–5 times for c – e . Shown is the representative data from one of the experiments. Error bars are mean ± standard error of the mean. ns not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Ova (10 μg) was purchased from Sigma-Aldrich (Cat No. A5503) and Chikungunya virus envelope 2 protein (8 μg) (CHIKV-E2, strain SL-CK1) was purchased from Sino Biological (Cat. No. 40440-V08B).

Techniques: Staining, Expressing